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Cooking measures for PDA culture Agar
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peterthinks
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Joined: Apr 18, 2004
Posts: 51

PostPosted: Fri Dec 10, 2004 10:04 am    Post subject: Reply with quote

Apparently they take over and collonize so fast than contaminants don't have a chance to start.
never tried it though....sounds plausible to me.

Peterthinks
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c
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PostPosted: Fri Dec 10, 2004 10:55 am    Post subject: Reply with quote

That might be a good way to go, if you have a lot of fruit bodies to spare! Although I can see it would be hard to use up a bunch of oysters instead of eating them.
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cultured1
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PostPosted: Sat Dec 11, 2004 12:01 am    Post subject: Reply with quote

I've heard of tissue suspension used as an innoculant too. I'm not sure if I'd take the risk of potentially adding contaminants to large ammounts of substrate. Maybe try it out on a small scale first.
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Funguy
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Location: BlueRidge Mountains , Virginia

PostPosted: Sat Dec 11, 2004 12:46 am    Post subject: Reply with quote

I have to agree that a whole mushroom seems most likely leed to contams.I just don't see how to clean it .Maybe a H2o2 bath would do it . I do understand the idea that the mycelium would excel and out grow the competitors . Due to the mycelium already being up and growing and the contams would have to germinate. I would be willing to try it .I have used water in a spawn jar and shaken hard, allowing the grain to bust the mycelium.Then used a syringe for innoculation in to compost .
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cultured1
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PostPosted: Sat Dec 11, 2004 6:01 am    Post subject: Reply with quote

Probably the best way would be to tear open a couple stems & use the tissue from the inside that isn't exposed to outside contaminants.
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