Just head for the grocery store...I've made clones from store bought fresh mushrooms.
Hmmmm... $30 for a culture slant from a mushroom supplier or $0.30 for a mushroom from the store?
I've never tried it(toilet paper) but I've been thinking about it.
I have about 5 pounds of grain grown thru with oyster that I'm trying to pin right now.Read the write up I did under Cultivating for cloning details.

It would be nice to create my own tissue cultures.
I want to try out a simple cheap grow kit first, as a test. Since I don't have all of the materials needed for a tissue culture.
I was given a pretty expensive gormet grow kit a few years ago as a gift. Sadly enough, I couldnt get the fool proof grow kit to fruit. It ended up turning a nasty color and I had to toss it :/

Well I'll send you a couple sterile agar slants if you like so you can take a culture of your grow kit's mushrooms...just in case you want to try it again in the future on your own.Private message me and We'll work out the details.
Here is a place with some pretty good kit prices.
www.gmushrooms.com/POTS.HTM

Peter

That would be really cool, thanks a lot for the offer. Sending a PM.
I will try to think of something to give you in return.

I also could clone some wild strains, I am still finding them here in the Northeast.

Heres what I use for my cultures, a modified MEA. Being a home brewer, I have some old malt extract laying around.
Percentages are weight/volume

3% malt extract
0.5% yeast extract
1.5% agar

The standard 20 minutes at 15psi to sterilize.

Sometimes I'll use water from grain soaking or straw pasteurization mixed 50 50 with distilled when mixing the agar, works great.

C1

Thanks Peter for the link for growing oyster mushroms on toilet rolls. Its similar to something I saw on a newsgroup a while back.

The guy on the newsgroup used some liquidised oyster mushroom(liquidising it in a sanitised food processor) and got some good results. Its something I'm thinking about doing over the winter. I also read that the toilet roll has to be non-bleached. Is this true??

Thanks, Mark